The Microscopy and Histopathology CoLab is an institutional shared research resource at SCRI that provides access to advanced cutting-edge imaging technology and tissue processing services in a collaborative manner for investigators at SCRI that is cost effective and equitable.
Microscopy:
Available microscopes in the CoLab include a Zeiss Microscopy LSM 900 confocal microscope with 405, 488, 561, and 640 nm laser lines, and a Zeiss Microscopy LSM 980 confocal microscope with 405, 445, 488, 514, 561, 594, 639, and 730 nm laser lines and NIR detectors. The two confocal microscopes are equipped with Airyscan 2 technology, which allows for super resolution imaging by harnessing an array of 32 area detector elements to collect additional information by improving the signal to noise ratio. Microscopy services also include training and support with image visualization and analysis.
Slide digitization services are provided using Zeiss Microscopy Axioscan 7 which has a capacity of up to 100 slides per scan using objectives that include 5x, 10x, 20x, and 40x magnification.
Starting July 2024, the CoLab will provide the following image analysis capabilities, available for scheduling in iLab:
Histopathology:
Histopathology services provided by the CoLab are include tissue processing, embedding, sectioning, and basic staining as well as multiplex immunohistochemistry. Consultation on experimental set up, slide preparation and data analysis are also available.
Aarya Vaikakkara Chithran, PhD - Research Scientist II (Microscopy)
Galen Stewart, BA - Research Technician III (Histology)
Ginny Schultz, MS - Research Lab Supervisor (Histology/Spatial Biology)
Monday-Friday 9:00 am - 5:00 pm | |
Microscopy: JMB 566A | Image analysis workstation in JMB 576 |
|
Name | Role | Phone | Location | |
---|---|---|---|---|
Aarya Vaikakkara Chithran, PhD |
Research Scientist II
|
206-884-4655
|
aarya.vaikakkarachithran@seattlechildrens.org
|
JMB 745
|
Galen Stewart, BA |
Research Technician III
|
galen.stewart@seattlechildrens.org
|
BC 08-134
|
|
Ginny Schultz, MS |
Research Lab Supervisor
|
206-884-4035
|
ginny.schultz@seattlechildrens.org
|
BC 08
|
Service list |
► IHC (1) | |||
Name | Description | Price | |
---|---|---|---|
Multiplex Immunohistochemistry (2-Plex) |
Price per mIHC for double stained slide. All IHC requests will be seen as special projects that require consultation with CoLab staff to discuss antibody optimization and project budgeting. |
Inquire |