Microscopy and Histopathology CoLab

Overview of Services

The Microscopy and Histopathology CoLab is an institutional shared research resource at SCRI that provides access to advanced cutting-edge imaging technology and tissue processing services in a collaborative manner for investigators at SCRI that is cost effective and equitable.

Microscopy:

Available microscopes in the CoLab include a Zeiss Microscopy LSM 900 confocal microscope with 405, 488, 561, and 640 nm laser lines, and a Zeiss Microscopy LSM 980 confocal microscope with 405, 445, 488, 514, 561, 594, 639, and 730 nm laser lines and NIR detectors. The two confocal microscopes are equipped with Airyscan 2 technology, which allows for super resolution imaging by harnessing an array of 32 area detector elements to collect additional information by improving the signal to noise ratio. Microscopy services also include training and support with image visualization and analysis.

Slide digitization services are provided using Zeiss Microscopy Axioscan 7 which has a capacity of up to 100 slides per scan using objectives that include 5x, 10x, 20x, and 40x magnification.

The CoLab also provides the following image analysis capabilities, available for scheduling in iLab:

1. Imaris 10.2 core facilities package with a floating license manager
2. Zeiss Zen 3.7 including LSM Plus and Airyscan Joint Deconvolution modules
3. Zeiss Arivis pro 4.1.0 (formerly Vision4D)
4. 3D Slicer including SlicerMorph extension

Histopathology:

Histopathology services provided by the CoLab are include tissue processing, embedding, sectioning, and basic staining as well as multiplex immunohistochemistry. Consultation on experimental set up, slide preparation and data analysis are also available.  A self-use cryostat and vibratome are also available for trained users (training provided by CoLab).

CoLab personnel

Aarya Vaikakkara Chithran, PhD - Research Scientist II (Microscopy)

Galen Stewart, BA - Research Technician III (Histology)

Ginny Schultz, MS - Research Lab Supervisor (Histology/Spatial Biology)

Location and hours of operation

Contacts