Microscopy and Histopathology CoLab

Overview of Services

The Microscopy and Histopathology CoLab is an institutional shared research resource at SCRI that provides access to advanced cutting-edge imaging technology and tissue processing services in a collaborative manner for investigators at SCRI that is cost effective and equitable.

Microscopy:

Available microscopes in the CoLab include a Zeiss Microscopy LSM 900 confocal microscope with 405, 488, 561, and 640 nm laser lines, and a Zeiss Microscopy LSM 980 confocal microscope with 405, 445, 488, 514, 561, 594, 639, and 730 nm laser lines and NIR detectors. The two confocal microscopes are equipped with Airyscan 2 technology, which allows for super resolution imaging by harnessing an array of 32 area detector elements to collect additional information by improving the signal to noise ratio. Microscopy services also include training and support with image visualization and analysis.

Slide digitization services are provided using Zeiss Microscopy Axioscan 7 which has a capacity of up to 100 slides per scan using objectives that include 5x, 10x, 20x, and 40x magnification.

Starting July 2024, the CoLab will provide the following image analysis capabilities, available for scheduling in iLab:

  1. Imaris 10.1 core facilities package with a floating license manager (starting July 11)
  2. Zeiss Zen 3.7 including LSM Plus and Airyscan Joint Deconvolution modules
  3. Zeiss Arivis pro 4.1.0 (formerly Vision4D)
  4. 3D Slicer including SlicerMorph extension

Histopathology:

Histopathology services provided by the CoLab are include tissue processing, embedding, sectioning, and basic staining as well as multiplex immunohistochemistry. Consultation on experimental set up, slide preparation and data analysis are also available. 

CoLab personnel

Aarya Vaikakkara Chithran, PhD - Research Scientist II (Microscopy)

Galen Stewart, BA - Research Technician III (Histology)

Ginny Schultz, MS - Research Lab Supervisor (Histology/Spatial Biology)

Location and hours of operation

Monday-Friday 9:00 am - 5:00 pm  

Microscopy: JMB 566A  | Image analysis workstation in JMB 576
Histopathology: Building Cure 08-550
Slide scanning sample drop off: JMB 745 (please coordinate drop off with CoLab staff beforehand)

 

Contacts

Name Role Phone Email Location
Aarya Vaikakkara Chithran, PhD
Research Scientist II
 
206-884-4655
 
aarya.vaikakkarachithran@seattlechildrens.org
 
JMB 745
 
Galen Stewart, BA
Research Technician III
 

 
galen.stewart@seattlechildrens.org
 
BC 08-134
 
Ginny Schultz, MS
Research Lab Supervisor
 
206-884-4035
 
ginny.schultz@seattlechildrens.org
 
BC 08
 

Service list


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