Genomics and Spatial Biology CoLab

The PacBio Revio is a 3rd generation sequencing platform using single molecule, real time (SMRT) sequencing to generate high quality consensus reads up to 20kb in length for gDNA. A single SMRTcell generates approximately 90GB of data, roughly 30x coverage of a human genome, with minimum phred score of Q30. This amplification-free DNA sequencing method also provides 5mC calls for every read, unlocking epigenetic information with no additional treatments or computation.

Revio sequencing can also be used for transcriptome & isoform sequencing, multiplexed PCR product or amplicon sequencing, and multiplexed microbial genome sequencing.

Sequencing-only includes the cost of 1 SMRTcell, sequencing reagents, and labor. Data will be returned as unaligned BAMs and PDF quality report by default, but users may opt to receive kinetic data as well.

Library QC (Qubit & FemtoPulse) are available for additional fee per prepared library.

The PacBio Revio is a 3rd generation sequencing platform using single molecule, real time (SMRT) sequencing to generate high quality consensus reads up to 20kb in length for gDNA. A single SMRTcell generates approximately 90GB of data, roughly 30x coverage of a human genome, with minimum phred score of Q30. This amplification-free DNA sequencing method also provides 5mC calls for every read, unlocking epigenetic information with no additional treatments or computation.

Revio sequencing can also be used for transcriptome & isoform sequencing, multiplexed PCR product or amplicon sequencing, and multiplexed microbial genome sequencing.

Sequencing-only includes the cost of 1 SMRTcell, sequencing reagents, and labor. Data will be returned as unaligned BAMs and PDF quality report by default, but users may opt to receive kinetic data as well.

Library QC (Qubit & FemtoPulse) are available for additional fee per prepared library.