Genomics and Spatial Biology CoLab

The PacBio Revio is a 3rd generation sequencing platform using single molecule, real time (SMRT) sequencing to generate high quality consensus reads up to 20kb in length for gDNA. A single SMRTcell generates approximately 90GB of data, roughly 30x coverage of a human genome, with minimum phred score of Q30. This amplification-free DNA sequencing method also provides 5mC calls for every read, unlocking epigenetic information with no additional treatments or computation.

Revio sequencing can also be used for transcriptome & isoform sequencing, multiplexed PCR product or amplicon sequencing, and multiplexed microbial genome sequencing.

Sequencing-only includes the cost of 1 SMRT Cell, sequencing reagents, and labor. Data will be returned as unaligned BAMs and PDF quality report by default, but users may opt to receive kinetic data as well.

Library QC (Qubit & FemtoPulse) are available for additional fee per prepared library.

The PacBio Revio is a 3rd generation sequencing platform using single molecule, real time (SMRT) sequencing to generate high quality consensus reads up to 20kb in length for gDNA. A single SMRTcell generates approximately 90GB of data, roughly 30x coverage of a human genome, with minimum phred score of Q30. This amplification-free DNA sequencing method also provides 5mC calls for every read, unlocking epigenetic information with no additional treatments or computation.

Revio sequencing can also be used for transcriptome & isoform sequencing, multiplexed PCR product or amplicon sequencing, and multiplexed microbial genome sequencing.

Sequencing-only includes the cost of 1 SMRT Cell, sequencing reagents, and labor. Data will be returned as unaligned BAMs and PDF quality report by default, but users may opt to receive kinetic data as well.

Library QC (Qubit & FemtoPulse) are available for additional fee per prepared library.