ABSL-3/BSL-3 Lab

Overview of Services

CGIDR has state-of-the-art M. tuberculosis (Mtb)-dedicated Biosafety Level 3 (BSL-3) and Animal Biosafety Level 3 (ABSL-3) laboratories fully equipped to ensure biocontainment and biosafety. The facilities are designed with a controlled-access anteroom, negative pressure, dedicated gas lines, shower and pass-through autoclave for operator safety.  The equipment for scientific research includes ultra-low freezers, fermentor for controlled growth of M. tuberculosis, CO2 incubators, centrifuges with aerosol containment, spectrophotometer, 96-well luminometer/fluorometer, bead-beater, refrigerators, freezers, sinks, microscopes and all other equipment needed to conduct Mtb research. This facilities have multiple individual research suites (with 8 Type II biosafety cabinets) that can be scheduled to maximize flexibility and facilitate containment. All parental strains of M. tuberculosis are already located within the BSL-3 laboratory.     

Animal health is monitored by a staff veterinarian and qualified animal care staff.  The animal program is overseen by the Center’s Institutional Animal Care and Use Committee (IACUC). The Center has an active Animal Welfare assurance on file with OLAW (A3640-01) and is AAALAC accredited.”                

The ABSL-3 and BSL-3 facilites are operated as a Core service on a fee per entry basis.  All  personal protective equipment (PPE) and basic lab supplies are provided, as well as service and maintenance on the equipment located in the facility.

Leadership

Christoph Grundner, Associate Professor

BSL-3 Director

(206) 884-3166, christoph.grundner@seattlechildrens.org

Location and hours of operation

Links and Resources

• TB BSL-3 Manual
• BSL-3 Training Matrix
• SCRI BSL-3 Website

Contacts